DMSO-PCR級(jí)
Dimethyl Sulfoxide Solution-PCR Grade
● 產(chǎn)品貨號(hào)及規(guī)格：
|
貨號(hào)
|
名稱
|
規(guī)格
|
貯存
|
|
RTE3102-02
|
DMSO溶液 PCR級(jí)
|
5×1 ml
|
-20℃
|
● 產(chǎn)品簡(jiǎn)介：
Betaine, DMSO and formamide helps in reducing the secondary
structure of GC rich templates and assists amplification of these templates.
Betaine is used at3.5Mto0.1M, DMSO should be used between 2-8%,
however 10% DMSO can reduce Taq polymerase activity by up to 50%. Formamide is
generally used at 1-5%. It has been our experience that the use of DMSO in
combination with betaine is superior to using formamide.
● 貯存：-20℃貯存。有效期二年。
● 使用說(shuō)明：
1．-20℃保存的DMSO常溫融化，混勻。
2. 根據(jù)根據(jù)PCR反應(yīng)體系，加入適量體積的DMSO溶液。
|
|
20 μl 體系
|
反應(yīng)體系終濃度
|
|
10×PCR Buffer
|
2 μl
|
1×
|
|
DMSO*
|
0.4-1.6 μl
|
2-8%
|
|
模板
|
0.05-0.5μg
|
as you wish
|
|
上游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
下游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
dNTP10mM
|
0.5 μl
|
200 μM
|
|
Taq
|
0.5-1 μl
|
2.5-5 U
|
|
滅菌水
|
up to 20 μl
|
|
注*：不同模板，不同片段的擴(kuò)增可能需要不同濃度的DMSO，可以適當(dāng)調(diào)節(jié)加入體積，找到適合自己反應(yīng)的甜菜堿濃度。
● References：
Frackman, S., Kobs,
G., Simpson, D. and Storts, D. 1998. Betaine and DMSO: enhancing agents for
PCR.
Promega
Notes 65: 27.